The main objective in poultry production is to achieve high quality products at high levels of productivity. The key for producers to achieve this goal is to avoid bacterial challenges e.g. from feed, water, environment, gastrointestinal tract and to mitigate mycotoxins. Doing so without the use of antibiotic growth promoters (AGPs) requires different strategies that focus on prevention and promote good gut performance. Traditionally, AGPs have been supplemented in poultry feed to improve performance and mitigate the negative effects of pathogens that colonise the gastrointestinal tract (GIT). Most of these pathogens are Gram-negative bacteria that have a lipopolysaccharide (LPS) or endotoxin layer. The release of LPS in an animal’s bloodstream can cause severe health problems in poultry and aggravate the effects of heat stress. Concerns about potential development of antibiotic resistance due to excessive and unregulated use of AGPs have led to regulations banning their use in Europe and consumer pressure with the same objective in North America. The ban on AGPs in poultry diets has been accompanied by an increased incidence of certain diseases, raising interest on alternatives...