The Cryo-EM Puck basic lab starter kit is ideal for those just starting to use cryo-electron microscopy (cryo-EM) and for smaller projects or labs. The kit helps with organizing, tracking, and shipping your samples on grids for single-particle analysis and other cryo-EM applications. It includes 10 MiTeGen 2nd Generation Cryo-EM Pucks; one cane for storing your pucks; one cane for shipping them; one grasping tong for handling them safely and securely; and Cryo-EM Puck barcoding and serialization for organizing and tracking your samples. Our kits can be customized as needed.

The BioContinuum imaging combines high-performance post-column energy filtration with direct detection technology. Because the filter is ideally suited for low-dose imaging applications, researchers can maximize their cryo-EM and cryo-electron tomography (cryo-ET) capabilities to gain further insight into system function and disease progression at the molecular level. The filter offers a 24-megapixel (5,760 × 4,092) field of view and works at 1,500 full frames per second. The BioContinuum comes with an optional, inline, GPU-based motion correction that saves researchers the need to save terabytes of raw frames.

JEOL announces the CRYO ARM 300 Field Emission Cryo-Electron Microscope for automatic, unattended acquisition of image data for single-particle analysis. The system is composed of an autoloading specimen stage (of up to four grids) cooled to liquid nitrogen temperatures and a cryo-storage device for long-term storage of up to 12 frozen-hydrated specimens. A cold field-emission gun produces a high-brightness electron beam with a very small energy spread, thus offering high temporal coherency. Equipped with an improved in-column energy filter (Omega filter), the CRYO ARM 300 acquires energy-filtered images and energy-loss spectra. The CRYO ARM 300 incorporates JEOL Automated Data Acquisition System (JADAS) automated software for single-particle analysis workflows. The hole-free phase plate consists of a thin, continuous carbon film placed in the back focal plane of the objective lens, providing a substantial improvement in image contrast of frozen-hydrated specimens.

Our "Introduction to Cryo-EM" Consumables Kit provides the basic requirements for getting started with grid preparation, and is perfect for both new and existing users looking to try different techniques, test suitability, and explore other product ranges. It includes a pack of R2/2 Quantifoils on 400-mesh copper grids. A variety of cryo grid boxes are supplied for grid storage; the round boxes are compatible with most plunge freezers, and the type of grid box chosen is usually based on personal preference. The AGG3729 squared box has also been included, which is compatible with the Gatan CT3500. The kit also includes three types of tweezers, electrostatic discharge (ESD) soft grip with extra fine tips, ESD epoxy-coated stainless steel with antimagnetic properties, and the component handling tweezers, which are useful for handling grid boxes with the screw type lid.

Rave Scientific offers a variety of high-quality, Swiss-made, precision cryo grid box handling tweezers, specially designed for handling the small cryo grid boxes used for storing transmission electron microscopy (TEM) grids under liquid nitrogen. These tweezers can be used with the EM-Tec GB-4 cryo grid box and other cryo grid boxes of similar design with a rotating lid and a central screw to hold the lid. They are designed for .05-mm screw heads but can also be used for screw-head sizes .03 mm–.06 mm. The length of these tweezers is 145 mm.

The SWISSCI Cryo-EM Grid Box is made from a special polymer formulation that ensures no static charging occurs at cryogenic temperatures. It has four diamond-shaped slot positions, each having its own number indicated on the side. No special tools are required, since the lid is rotated with standard laboratory tweezers. Individually numbered Pressure Adhesive Cryo Labels are supplied with the boxes, giving the consumer a convenient alternative to marking up each box themselves. The Cryo-EM Grid Box fits all common cryo-EM sample mounting and storage devices. Its low cost enables single use, giving each stored sample a unique identification number and preventing sample mix-ups.

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A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.

A weekly roundup of information on newly offered instrumentation, apparatus, and laboratory materials of potential interest to researchers.

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